Coding

Part:BBa_K112117:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)


a~BglII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

PCR mv027/mv010 on pBca9145-Bca1147    (707bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112117
-----------------------------------------------
mv027	Forward Biobricking of a~r.BglII!
ccataGAATTCatgAGATCTcataatgaagattgatataacggactataaccatgc
mv010	Reverse Biobricking of r.BglII!
cgttaGGATCCttaatatgtcacgattgttcctc

References